Fig. 7: An energetic basis for utilizing diverse electron shuttles during SAO by DTU068_1.
Free energy yields (ΔG) per mole of ATP produced for each member of the syntrophic acetate oxidizing consortium as a function of hydrogen partial pressure (PH2), for the cases where the Methanothermobacter_2 MAG (A) is present; and (B) is not present. The dashed gray line represents the ATP phosphorylation potential measured in cells performing acetogenesis from H2 and CO2 (−32.1 kJ/mol-ATP) [96]. Shaded regions represent the ranges of hydrogen partial pressure that would support ATP synthesis by DTU068_1 and Methanothermobacter_1 (yellow region) or DTU068_1 and Methanothermobacter_2 (red region). The free energy values were calculated based on reaction stoichiometry predicted by the parsimonious flux balance analysis model for both cases (Supplementary Data 2), assuming environmentally-relevant concentrations of acetate (50 mM), formate (7.5 μM), methane (0.5atm) and carbon dioxide (0.5atm).
