Table 3 Primers used for cloning Tmem132d promoter fragments into the luciferase vector
Primer | Orientation | Primer sequence (5’ → 3’) |
|---|---|---|
RVprimer3 | Forward | CTAGCAAAATAGGCTGTCCC |
GLprimer2 | Reverse | CTTTATGTTTTTGGCGTCTTCCA |
Tmem132d | Forward | GCAGGTACCCAAGGCTCTGCGGAGCAGTG |
Tmem132d | Reverse | GCTAGCAATTTCTCTCTCTTCCTCTCTCCC |
Tmem132dreverse310 A sub | Forward | GTTAGGGGTTCCTGAACTGTCCTTGCCTGAAG |
Tmem132dreverse310 A sub | Reverse | CTTCAGGCAAGGACAGTTCAGGAACCCCTAAC |
Tmem132dreverse310 del | Forward | GACGGTACCTCAGGGACAGGAATTTGAGG |
Tmem132dreverse519 A sub | Forward | GTGTGAGTTCGCCTTAGATACCCTGGAAGG |
Tmem132dreverse519 A sub | Reverse | CCTTCCAGGGTATCTAAGGCGAACTCACAC |
