Fig. 3: NAP protects against increases in hippocampal MD and FA in the Adnp^+/− mice.

Two-way ANOVA with Tukey post-hoc test was performed (n = 4–6 per experimental group). a For mean diffusivity (MD), a representative T2-weighed image at the level of the hippocampus at −1.84 mm from Bregma is presented. Main genotype (F(1,16) = 8.775, p = 0.009) and interaction (F(1,16) = 4.956, p = 0.041) effects were found, with a significant increased MD in chlorobutanol (CB)-treated Adnp^+/− mice, as compared with their Adnp^+/+ control mice (**p < 0.01). This increase, although insignificant, was slightly reduced in NAP-treated Adnp^+/− mice. b For fractional anisotropy (FA), a representative T2-weighed image at the level of the hippocampus at −2.34 mm from Bregma is presented. Main treatment (F(1,16) = 12.782, p = 0.003) and interaction (F(1,16) = 9.986, p = 0.006) effects were found, with a significant increased FA in chlorobutanol (CB)-treated Adnp^+/− mice, as compared with their Adnp^+/+ control mice (*p < 0.05). This increase was significantly reduced in NAP-treated Adnp^+/− mice (***p < 0.001)