Fig. 1: Stimulation of an AgRP neuron triggers sEPSC LTD in its downstream POMC neuron.
From: AgRP neurons trigger long-term potentiation and facilitate food seeking
A Representative microscopic images showing GFP-labelled AgRP neurons (left), tdTOMATO-labelled POMC neurons (middle), and merge (right) from NPY-GFP/POMC-CreERT2/Rosa26-LSL-tdTOMATO mice. B, C Representative action potential traces from an AgRP neuron and a POMC neuron that do not form a synapse (B) or from a pair that forms a synapse (C). D, E Representative traces for IPSCs recorded from a POMC neuron upon a positive current injected into its pre-synaptic AgRP neuron, in the absence (D) or the presence (E) of 4-AP and TTX. F, G Quantifications of IPSC amplitude (F) and latency (G). Data are mean ± SEM. n = 7 per group. ***P < 0.001 vs. 1st group; ###P < 0.001 vs. 2nd group in one-way ANOVA analyses followed by Tukey’s tests. H Schematic protocol to induce sEPSC LTD at the AgRP→POMC synapse. I Upper panel: representative sEPSC traces at the baseline and after AgRP neuron stimulation recorded from the POMC neuron that was synapsed by the AgRP neuron. Lower panel: quantitative changes in sEPSC amplitudes (as % of the baseline) in POMC neurons before and after the 10-min AgRP neuron stimulation. Data are mean ± SEM. n = 6 per group. J Upper panel: representative sEPSC traces at the baseline and after AgRP neuron stimulation recorded from the POMC neuron that was not synapsed by the AgRP neuron. Lower panel: quantitative changes in sEPSC amplitudes (as % of the baseline) in POMC neurons before and after the 10-min AgRP neuron stimulation. Data are mean ± SEM. n = 6 per group. Also see Fig. S1.
