Fig. 2: Characterization of the sEPSC LTD in AgRP-innervated POMC neurons.
From: AgRP neurons trigger long-term potentiation and facilitate food seeking
A Temporal changes in sEPSC amplitudes in AgRP-innervated POMC neurons from mice that were fed ad libitum or fasted for 24 h. B The average sEPSC amplitude after AgRP stimulation in (A). Data are mean ± SEM. n = 6 per group. ***P < 0.001 in two-tailed unpaired t tests. C Temporal changes in sEPSC amplitudes in AgRP-innervated POMC neurons (from fasted mice) after the brain slices were pre-incubated with vehicle, leptin, or insulin. D The average sEPSC amplitude after AgRP stimulation in (C). Data are mean ± SEM. n = 5 or 6 per group. ***P < 0.001 vs. the no treatment group in one-way ANOVA analyses followed by post hoc Tukey’s tests. E, F Amplitudes of NMDA (E) and AMPA (F) sEPSC in POMC neurons before (baseline) and after (LTD) the 10-min AgRP neuron stimulation. Data are mean ± SEM. n = 6 per group. **P < 0.01 and ***P < 0.001 in two-tailed paired t tests. G Temporal changes in sEPSC amplitudes in POMC neurons before and after the 10-min AgRP neuron stimulation (from fasted mice) after the brain slices were pre-incubated with various blockers. H The average sEPSC amplitude after AgRP stimulation in (G). Data are mean ± SEM. n = 6 or 7 per group. **P < 0.01 and ***P < 0.001 vs. the no blocker group in one-way ANOVA analyses followed by post hoc Tukey’s tests. Also see Fig. S1.
