Fig. 6: The AgRP→ PVT circuit regulates food seeking.
From: AgRP neurons trigger long-term potentiation and facilitate food seeking
A Schematic illustration to selectively ablate AgRP neurons projecting to the PVT. B Number of entries to food-coupled arm by control and AgRPPVT-Ablation mice during pre-test and the test in the Y-maze assay. n = 7 or 12 per group. *P < 0.05 between pre-test and the test in two-way ANOVA repeated measurement followed by post hoc Sidak tests. Note that the number of data points may appear fewer than the n values because multiple mice showed the same number of entries during the pre-test or the test. C, D Incorrect (C) and correct pokes (D) of control and AgRPPVT-Ablation mice in the food-baited hole board test. Data are mean ± SEM. n = 12 or 15 per group. *P < 0.05 vs. control in one-tailed unpaired t tests. E Schematic illustration to selectively inhibit AgRP→PVT projections. F Number of entries to the food-coupled arm in the pre-test and the test in the Y-maze assay by control mice and mice with inhibited AgRP→PVT projections. n = 10 per group. *P < 0.05 between the pre-test and the test in two-way ANOVA repeated measurement followed by post hoc Sidak tests. Note that the number of data points may appear fewer than the n values in because multiple mice showed the same number of entries during the pre-test or the test. G Schematic illustration to selectively activate AgRP→PVT projections. H Number of entries to the food-coupled arm in the pre-test and the test in the Y-maze assay by control mice and mice with activated AgRP→PVT projections. n = 10 per group. Note that the number of data points may appear fewer than the n values in because multiple mice showed the same number of entries during the pre-test or the test. Also see Figs. S6, S7.
