Fig. 2: Adiponectin enables CLSP to be active in the presence of higher concentrations of apolipoprotein E4.
From: Restoration of the reduced CLSP activity alleviates memory impairment in Alzheimer disease
a–c SH-SY5Y cells, transfected with the empty vector or pcDNA3.1/MycHis-V642I-APP, were cultured in media containing 1 nM of GST-MycHis or CLSP-MycHis with 10 nM of ApoE3 or BSA with 10 nM of adiponectin (ADN) (a), annexin 2 (b), annexin 5(c), or BSA. GST-MycHis and BSA were used as negative controls. At 48 h, cells were harvested for trypan blue exclusion assays. d SH-SY5Y cells, transfected with the vector or pcDNA3.1/MycHis-V642I-APP, were cultured in media containing 1 nM of GST-MycHis or CLSP-MycHis with/without 10 nM of ApoE4 with/without indicated concentrations of adiponectin (ADN). At 48 h, the cells were harvested for the trypan blue exclusion and calcein assays. e SH-SY5Y cells, transfected with the vector or pcDNA3.1/MycHis-V642I-APP, were cultured in media containing 1 nM of GST-MycHis or CLSP-MycHis with/without stepwise increasing concentrations of ApoE4 with/without 1 nM of ADN. At 48 h, the cells were harvested for trypan blue exclusion and WST-8 assays. The cell lysates were immunoblotted using the APP antibody.
