Fig. 4: Methylation of the PSORS1C3 cloned pCpGL vector significantly reduces luciferase activity.
From: Genome-wide DNA methylation meta-analysis in the brains of suicide completers
There is a significant increase in firefly luciferase expression when normalised to Renilla luciferase, the normalised expression ratios of firefly activity increases to 3.81 when unmethylated versus 0.0269 when methylated in vitro (Students t-test, P = 0.0057, n = 3) which corresponds to a 206-fold change increase in luciferase activity in the unmethylated PSORS1C3 cloned vector compared to the methylated identical vector. N = 3 experimental repeats with each experiment containing three technical repeats. Error bars represent ± the standard error.
