Fig. 2: Effects of SPS&S procedure on relative protein expression normalized by β-actin and synaptic ultrastructure in the PFC and Hip and levels of cortisol and oxytocin in blood. | Translational Psychiatry

Fig. 2: Effects of SPS&S procedure on relative protein expression normalized by β-actin and synaptic ultrastructure in the PFC and Hip and levels of cortisol and oxytocin in blood.

From: The potential role of the HCN1 ion channel and BDNF-mTOR signaling pathways and synaptic transmission in the alleviation of PTSD

Fig. 2

a–c The expression of HCN1 (Student’s t-test: t = −6.255, p = 0.001), BDNF (t = 3.805, p = 0.009) and pmTOR (t = 4.226, p = 0.006) in PFC. g–i The expression of HCN1(Student’s t-test: t = −3.579, p = 0.012), BDNF (t = 3.733, p = 0.022) and pmTOR (t = 3.424, p = 0.014) in Hip. d–f, j–l Localization and distribution of HCN1, BDNF and pmTOR in PFC (d–f) and (j–l). Bar = 20 μm. o–q The PSD (Student’s t-test: t = 6.499, p = 0.001), curvature of synaptic interface (t = 7.021, p = 0.003) and width of synaptic cleft (t = −2.628, p = 0.039) in the PFC. t–v The PSD (Student’s t-test: t = 1.846, p = 0.158), curvature of synaptic interface (t = 0.499, p = 0.635) and width of synaptic cleft (t = 2.257, p = 0.065) in the Hip. m, n, r, s Synaptic ultrastructure in PFC (m, n) and Hip (r, s) under ×10,000 magnification. w, x The level of cortisol (Student’s t-test: t = −5.694, p = 0.000) and oxytocin (t = −6.508, p = 0.000) in blood. y Immunoreactivity analysis of HCN1 (Student’s t-test: t = 3.147, p = 0.0346), BDNF (t = 5.227, p = 0.006), and pmTOR (t = 6.184, p = 0.003) in PFC. z Immunoreactivity analysis of HCN1 (Student’s t-test: t = 21.82, p = 0.000), BDNF (t = 9.956, p = 0.001), and pmTOR (t = 11.23, p = 0.000) in Hip. Data are represented as mean ± SEM. *p < 0.05 was expressed statistically significant. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with control group.

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