Fig. 1: Physiological characterization of Ndufs4GT/GT mice.
a Relative Ndufs4 mRNA expression normalized to B2m and Gapdh using the Pfaffl method in the brain of WT (orange bars) and Ndufs4GT/GT (blue bars) mice. b NDUFS4 protein abundance relative to beta-Actin in the brain. c Z-scores of mitochondrial complex I (CI) activity from hippocampal tissue. d Body weight as measured after 20 days of chronic unpredictable stress (CUS). e Body weight change from the start of chronic stress to the end of the experiment. In this graph, the genotype is not shown since there was no difference in body weight change between WT and Ndufs4GT/GT mice over time. f Adrenal weight expressed as a percentage of the body weight of the animals. g Plasma corticosterone (CORT) levels measured one day after the last stressor. h Blood glucose concentrations measured with a FreeStyle Freedom Lite glucose meter using a drop of trunk blood shortly after decapitation. i Representative images from one animal showing the fractional anisotropy (FA) and mean diffusivity (MD) at different Bregmata. Approximately at Bregma 1.10 mm, 0.62 mm, −0.70 mm, −1.70 mm, −2.80 mm, and −6.24 mm. j FA was measured in several different brain regions. k In these same regions, MD was also measured. Graphs show the geometric mean (a) or average (b–h, j, k) ± SEM, with each black dot representing the result of an individual animal. *p < 0.05, **p < 0.01, and ***p < 0.001. Abbreviations: CC, corpus callosum; CP, cerebral peduncle; IC, internal capsule; AC, anterior commissure; CPu, caudate putamen; dHip, dorsal part of the hippocampus; vHip, ventral part of the hippocampus; Mid, midbrain; CTX, cortex; Sub, subthalamic regions; Cereb, cerebellum.
