Fig. 4: Embryonic offspring neurobiology was altered by maternal AVP.
From: Altered offspring neurodevelopment in an arginine vasopressin preeclampsia model
a The embryonic day (E) 18 dorsal forebrain was trend-smaller in AVP-condition offspring (males: p = 0.054, females: p = 0.086), which reflected (b) symmetrical growth restriction relative to body weight decrements with AVP exposure. c Dorsal forebrain cell density significantly increased with AVP exposure in male and female offspring [males: p = 0.009, females: p = 0.035] such that (d) the total forebrain cell number was unchanged. These volume differences were not accounted for by (e) changes in cortical vessel diameter or (f) cell size across the cortical plate (CP), intermediate (IZ), or ventricular (VZ) zones. g The TBR1-labeled cortical plate volume, (h) cell density, (i) and cell number were unchanged with AVP. j The volume of the intermediate, subventricular, and ventricular zones (IZ + SVZ + VZ) was significantly smaller in E18 AVP-exposed male offspring and trend-smaller in AVP-exposed female offspring (males: p = 0.005, females: p = 0.089), (k) also reflected by a decreased proportion of dorsal forebrain occupied by IZ + SVZ + VZ in AVP-exposed males (p = 0.001). *p < 0.05, **p < 0.01 per two-sample t-test, error bars represent SEM. 3 litters per condition, 1–3 brains per sex per litter.
