Fig. 1: Social discrimination tests conducted with adult C57BL\6J male mice.
A Schematic depiction of the social preference (SP) test. B Mean investigation time measured separately for each stimulus (20-s bins) across the SP test (n = 20). C Mean investigation time summed separately for each stimulus throughout the SP test. D–F As in A–C, respectively, for the social novelty preference (SNP; n = 20) test. G–I As in A–C, respectively, for the sex preference (SxP; n = 25) test. J–L As in A–C, respectively, for the emotional state preference (ESP; n = 14) test. (M–P) Mean investigation time for each of the stimuli, pooled separately for short (<7 s) and long (≥7 s) investigation bouts, for the SP (M), SxP (O), and ESP (P) tests shown above (A–L). Note that the preference for a specific stimulus is reflected only by the long bouts in all of these tests. Post hoc paired t-tests following main effect in mixed-model ANOVA test; M- short bouts: t19 = −0.576, p = 0.571, long bouts: t19 = −4.64, p < 0.001; N- short bouts: t19 = −0.93, p = 0.364, long bouts: t19 = 2.78, p < 0.05; O-short bouts: t24 = 0.973, p = 0.34, long bouts: t24 = 4.825, p < 0.001; P-short bouts: t13 = 0.708, p = 0.492, long bouts: t13 = 3.167, p < 0.01. *p < 0.05, **p < 0.01, ***p < 0.001, paired t-test following the main effect in ANOVA test. All error bars represent SEM.
