Fig. 1: PPI paradigm increases c-Fos expression of PV+ neurons in audTRN.
From: Involvement of the thalamic reticular nucleus in prepulse inhibition of acoustic startle
A Strategy of retrograde labeling of audTRN neurons projecting to MG. Retrobeads (RBs) were injected into MG of C57 mice. B Left, example of confocal image showing overlap of retrobeads-labeled neurons(red) and PV-positive neurons (green). White dotted lines mark the border of the TRN. Middle and right, enlarged images from the yellow box region in the left image. Arrows: dual-positive neurons. Scale bars: left to right, 100 µm, 40 µm. C Quantification shows that approximately 88.0% of RBs labeled cells are PV+ (N = 7 slices of 3 mice). D, F Schematic of the behavioral test before c-Fos staining experiment. C57 mice were put into the startle reflex chamber without sound stimulation (D, control group), or with prepulse inhibition (PPI) paradigm (F, PPI group). E, G Left, example of confocal image showing overlap of c-Fos-positive neurons and PV-positive neurons of control group (E), or PPI group (G). White dotted lines mark the border of the TRN. Middle and right, enlarged images from the yellow box region in the left image. Arrows: dual-positive neurons. Scale bars: left to right, 100 µm, 40 µm. H PPI paradigm increased c-Fos-positive neuron number compared with the control group (Mann–Whitney two-tailed t-test, P < 0.0001, Ncontrol = 7 slices of 3 mice, NPPI = 15 slices of 6 mice). I Quantification shows that approximately 89.38% of c-Fos-positive cells are PV+ (N = 15 slices of 6 mice, PPI group). ****P < 0.0001.
