Fig. 1: Generation of VB6-deficient mouse model and their behavioral abnormalities. | Translational Psychiatry

Fig. 1: Generation of VB6-deficient mouse model and their behavioral abnormalities.

From: Vitamin B6 deficiency hyperactivates the noradrenergic system, leading to social deficits and cognitive impairment

Fig. 1

VB6(−) mice were generated by feeding with a VB6-lacking diet from 8 to 12 weeks of age. A Changes in body weight during feeding with a VB6-lacking diet are shown. Two-way ANOVA with repeated measurements: FInteraction(4,132) = 7.15, p < 0.001; FDay(4,132) = 46.3, p < 0.001; FVB6(1,33) = 9.82, p < 0.01 (Control: n = 18; VB6(−): n = 17). *p < 0.05, **p < 0.01, and ***p < 0.001 using Bonferroni’s multiple comparison test. B Food intake of a control and VB6-lacking diet were measured during the first 1 week of the feeding. n.s. (not significant) using Student’s t-test. C VB6 level in mouse plasma was determined after feeding for 4 weeks. ***p < 0.001 by Student’s t-test. D PLP and E PMP in the brains of VB6(−) mice were quantified by HPLC. Two-way ANOVA: D FInteraction(3,30) = 3.58, p < 0.05; FArea(3,30) = 38.0, p < 0.001; FVB6(1,10) = 76.3, p < 0.001 and E FInteraction(3,30) = 0.53, p > 0.05; FArea(3,30) = 30.8, p < 0.001; FVB6(1,10) = 1.32, p > 0.05 (n = 6). n.s., *p < 0.05, **p < 0.01, and ***p < 0.001 using Bonferroni’s multiple comparison test. In social interaction test, F time spent in the chamber and G interaction time were measured (Control: n = 18; VB6(−): n = 17). In the novel object recognition test, H exploratory time and I exploratory preference were determined (Control: n = 20; VB6(−): n = 20). Two-way ANOVA: F FInteraction(1,33) = 4.07, p > 0.05; FSession(1,33) = 19.9, p < 0.001; FVB6(1,33) = 4.28, p < 0.05, G FInteraction(1,33) = 4.94, p < 0.05; FSession(1,33) = 39.2, p < 0.001; FVB6(1,33) = 2.88, p > 0.05, H FInteraction(1,38) = 8.10, p < 0.01; FSession(1,38) = 18.2, p < 0.001; FVB6(1,38) = 5.41, p < 0.05, and I FInteraction(1,38) = 0.02, p > 0.05; FSession(1,38) = 217, p < 0.001; FVB6(1,38) = 0.55, p > 0.05. *p < 0.05 and ***p < 0.05 using Bonferroni’s multiple comparison test. ###p < 0.001 and $p < 0.05 compared with control and VB6(−) mice in the habituation session respectively, by Bonferroni’s multiple comparison test. The data were represented as mean ± standard error of the mean (SEM). PFC prefrontal cortex, NAC nucleus accumbens, STR striatum, HIP hippocampus.

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