Fig. 1: Cyfip1 haploinsufficiency increases neurogenesis in vivo in the absence of changes in proliferation. | Translational Psychiatry

Fig. 1: Cyfip1 haploinsufficiency increases neurogenesis in vivo in the absence of changes in proliferation.

From: Haploinsufficiency of the schizophrenia and autism risk gene Cyfip1 causes abnormal postnatal hippocampal neurogenesis through microglial and Arp2/3 mediated actin dependent mechanisms

Fig. 1

A Experimental scheme, proliferation rates were studied with a 6 h pulse of BrdU. B Representative immunohistochemistry showing distribution and location of proliferative (Ki67+) and dividing (Ki67+BrdU+) cells in wild-type and Cyfip1+/− animals (n = 4 each), respectively. C Quantification showed no difference in numbers of proliferative cells or number of cell divisions. D Representative staining for DCX in wild-type and Cyfip1+/− animals (n = 8 each), respectively. E Quantification showed a significantly larger number of immature neurons in the Cyfip1+/− animals. F Experimental scheme, cell maturation was studied with a 30 day BrdU pulse-chase. G Representative images showing BrdU+ and NeuN+ cells. Arrowheads indicate BrdU+ cells H Cyfp1+/− animals (n = 6) had significantly higher numbers of BrdU+NeuN+ cells than the wild-type animals (n = 5). l The fraction of BrdU+ cells which had matured into NeuN+ cells was significantly increased in the Cyfip1+/− animals. All data are shown as mean ± SEM.

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