Fig. 4: Altered migration of immature Cyfip1+/− neurons. | Translational Psychiatry

Fig. 4: Altered migration of immature Cyfip1+/− neurons.

From: Haploinsufficiency of the schizophrenia and autism risk gene Cyfip1 causes abnormal postnatal hippocampal neurogenesis through microglial and Arp2/3 mediated actin dependent mechanisms

Fig. 4

A Immunohistochemistry for the Ki67, showing cells in the cell cycle, shows the majority are located in the SGZ (closed arrowheads), but some are starting to move out into the granular layer (open arrowheads). B A significantly larger fraction of Ki67+ cells are located in the SGZ in Cyfip1+/− animals, comparted to wild types. C The distance moved from the SGZ is significantly lower in the Cyfip1+/− animals, comparted to wild types. D Post-mitotic adult-born neurons (NeuN+/BrdU+) are located predominantly in the granular layer (open arrowheads), but some are still located in or near the SGZ (closed arrowheads). E As with Ki67+ cells, a smaller fraction of Cyfip1+/− cells are in the granular layer. F Distance moved by these cells is significantly lower. G Time-lapse imagining shows the movement of cells in primary hippocampal cultures. H Quantification of cell movement during live imaging shows a significantly smaller distance moved by the Cyfip1+/− cells.

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