Fig. 5: Luciferase reporter assays comparing transcriptional activation with pGL3-Promoter or variant alleles of genetic variants.
From: Genetic risk of clozapine-induced leukopenia and neutropenia: a genome-wide association study
a) rs377360. b) rs4773794. c) rs9808117. d) rs8024434. e) rs7501702. HEK-293 cells were transfected with luciferase reporter constructs for both alleles of each variant. Each construct was transfected five times and assayed in quintuplicate in each experiment. The luciferase activities, normalized with a cotransfected Renilla activities, were expressed by taking the normalized luciferase activity of the vector (pGL3-Promoter) to be 1. Data represent means±SDs. P values were determined by using the t-test. Significance indicators will be written above the bars: *** if p < 0·001, ** if p < 0·01, * if p < 0·05,ns otherwise.
