Fig. 1: Identification of genes affected by VPA exposure in hFOs.
From: Human forebrain organoids reveal connections between valproic acid exposure and autism risk
A Workflow of this study. B Immunofluorescence characterization for hFOs. Ventricular zone marker, SOX2 and ki67; intermediate zone marker, TBR2; cortical plate markers, MAP2; forebrain-specific markers, FOXG1 and PAX6. Scale bar, 50 μm. C Transcriptome correlation analysis between hFOs (day 45) and human fetal brains. PCW post-conception weeks. The color bar indicates the correlation coefficient. D Principal component analysis for RNA-seq data from VPA-exposed and control hFOs. E Overlap between VPA-induced DEGs identified in U1M and U2F hFOs. Among the 1242 overlapped genes, the expression change directions of COMT in U1M and U2F were not consistent. The gene COMT was not considered as a VPA-induced DEG, the remaining 1241 shared VPA-induced DEGs between U1M and U2F hFOs.
