Fig. 3: Analysis of the transcriptional drive of hormone response elements (HRE) in BRD1CRISPRex6/+ cells.
From: The psychiatric risk gene BRD1 modulates mitochondrial bioenergetics by transcriptional regulation
A Domains and nuclear receptor binding sites in BRD1. Top: Enhancer of polycomb-like, N-terminal domain (EPL1); Plant homeodomain finger (PHDZnF); Bromodomain (Bromo); Pro-Trp-Trp-Pro (PWWP). Bottom: Amino acid sequences (one-letter code) containing putative nuclear receptor (NR) binding sites (4 co-activators (LXXLL) and 1 co-repressor (LXXIXXL)). Pink letters indicate the putative NR binding sites. Amino acid numbering according to BRD1-S. B Transcription from promoters containing HREs recognized by respectively: HNF4 (hepatocyte nuclear factor); LXR (liver receptor); RXR (retinoid X receptor); PGR (progesterone receptor); GR (glucocorticoid receptor); VDR (vitamin D receptor); RAR (retinoic acid receptor); PPAR (peroxisome proliferator-activated receptor); AR (androgen receptor); and ESR (estrogen receptor) as well as a TATA box promoter (negative control (neg)) was tested in four distinct BRD1CRISPRex6/+ and four WT HEK cell lines by a dual luciferase-based array. P-value as determined by Student’s t-tests. p < 0.05 (*). Transcription from the PPAR HRE (PPRE)-containing promoter was near significantly increased (p = 0.085) in BRD1CRISPRex6/+ cells.
