Fig. 2: Results of qPCR and fluorescence in situ hybridization (FISH) experiments on miR-195a-5p expression in two different AD mouse models compared to controls.
From: Differential microRNA expression analyses across two brain regions in Alzheimer’s disease
a Expression of miR-195a-5p in entorhinal cortex of 6-month-old P301S mice compared to wild-type (WT) mice was assessed by qPCR (n = 4 for each group). *p-value from unpaired Student’s t test was used comparing transgenic vs. WT mice. b, c Expression of miR-195a-5p in hippocampus and temporal cortex of 6-month-old P301S mice compared to WT mice; no significant differences were identified. d Fluorescence in situ hybridization (FISH) images of miR-195a-5p which is stained green in EC of 6-month-old P301S and WT mice. The nuclei were stained blue with DAPI. Cabl1 (stained in red) was used as a marker of EC layer II. Scale bar indicates 20 μm. n = 3 mice in each group. e–h Analogous experiments comparing EC (e), hippocampus (f) and temporal g cortex and performing FISH staining (h) in 6-month-old APP/PS1 mice compared to WT mice.
