Fig. 3: Reduced intrinsic excitability of VC-L5 neurons in P28–32 5XFAD mice.

A VC-L5 neurons from 5XFAD brain slices show similar membrane input resistance (WT, n = 8 cells/5 mice; 5XFAD, n = 9 cells/6 mice. t₁₅ = 0.96, p = 0.35) and membrane capacitance (WT, n = 10 cells/5 mice; 5XFAD, n = 9 cells/6 mice. t₁₇ = 0.48, p = 0.63) compared to WT littermate L5 neurons. Representative current responses to voltage steps are on the top, based on which membrane properties are calculated. B VC-L5 neurons from 5XFAD mice exhibit similar action potential half-width (WT, n = 8 cells/5 mice; 5XFAD, n = 7 cells/6 mice. t₁₃ = 1.22, p = 0.25) and AP threshold (WT, n = 9 cells/5 mice; 5XFAD, n = 8 cells/6 mice. t₁₅ = 0.53, p = 0.61). C Representative action potential density plot from 5XFAD and WT VC-L5 neurons. Intrinsic excitability responses, measured by AP firing in response to current step injections (−100 to 500pA, with 50pA increment), was shown to the right. VC-L5 neurons from 5XFAD slices show reduced AP number in response to current injections (WT, n = 6 cells/5 mice; 5XFAD, n = 6 cells/5 mice. Repeated measures two-way ANOVA, genotypes effects: F_(1,10) = 9.6, p = 0.011). A significantly lower AP firing at higher current steps (350–500pA) was observed (Sidak’s post hoc multiple comparison test. **p < 0.01, ****p < 0.0001). D 5XFAD VC-L5 neurons show similar spike frequency adaptation compared to WT neurons (WT, n = 7 cells/5 mice; 5XFAD, n = 8 cells/7 mice. t₁₃ = 0.26, p = 0.80).