Fig. 3: Reduced activity of BF cholinergic neurons by Cul3 ablation. | Translational Psychiatry

Fig. 3: Reduced activity of BF cholinergic neurons by Cul3 ablation.

From: Cholinergic neurons in the basal forebrain are involved in behavioral abnormalities associated with Cul3 deficiency: Role of prefrontal cortex projections in cognitive deficits

Fig. 3: Reduced activity of BF cholinergic neurons by Cul3 ablation.

A, B Unchanged numbers of cholinergic neurons in both STR and BF of ChatCRECul3F/+ mice (STR: n = 22 slices/6 Cul3F/+ mice, n = 23 slices/6 ChatCRECul3F/+ mice, t(43) = 1.43, p = 0.16; BF: n = 13 slices/4 Cul3F/+ mice, n = 16 slices/4 ChatCRECul3F/+ mice, t(27) = 0.22, p = 0.83, unpaired two-tailed t-test). C Plot of synaptic-driven, spontaneous action potential (sAP) frequencies showing the significant reduction in BF cholinergic neurons of ChatCRECul3F/+ mice (n = 9 cells/3 Cul3F/+ mice, n = 13 cells/4 ChatCRECul3F/+ mice, t(20) = 2.85, p = 0.0098, unpaired two-tailed t-test). Inset: Representative sAP traces. D, E Representative confocal images and quantification of rpS6-S240/S244 in ChAT+ cholinergic neurons in the BF (D) or the STR (E) (D, n = 70 cells/6 Cul3F/+ mice, n = 67 cells/6 ChatCRECul3F/+ mice, t(10) = 5.69, p = 0.002; E, n = 68 cells/6 Cul3F/+ mice, n = 54 cells/5 ChatCRECul3F/+ mice, t(9) = 1.42, p = 0.19, unpaired two-tailed t-test). In all figures, **p < 0.01, ***p < 0.001.

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