Fig. 5: Chemogenetic activation of BF cholinergic neurons in ChatCRECul3F/+ mice ameliorates the PPI and cognitive abnormalities. | Translational Psychiatry

Fig. 5: Chemogenetic activation of BF cholinergic neurons in ChatCRECul3F/+ mice ameliorates the PPI and cognitive abnormalities.

From: Cholinergic neurons in the basal forebrain are involved in behavioral abnormalities associated with Cul3 deficiency: Role of prefrontal cortex projections in cognitive deficits

Fig. 5

A Schematic representation of AAV-hM3D(Gq) DREADD injection into the BF of ChatCRECul3F/+ mice. B Unchanged social interaction time in 3-chamber social preference tests after CNO activation of BF cholinergic neurons in ChatCRECul3F/+ mice (Cul3F/+: n = 10, ChatCRECul3F/+: n = 11, interaction time: Finteraction(3, 76) = 2.96, p = 0.037; Fzone(1, 76) = 125.1, p < 0.0001; Ftreatment(3, 76) = 2.23, p = 0.09; preference index: Finteraction(1, 38) = 3.39, p = 0.073; Fgenotype(1, 38) = 6.2, p = 0.017, Ftreatment(1, 38) = 2.68, p = 0.11; two-way ANOVA). C PPI deficit is ameliorated by CNO activation of BF cholinergic neurons in ChatCRECul3F/+ mice (Cul3F/+: n = 10, ChatCRECul3F/+: n = 11, Startle: Finteraction(9, 108) = 1.54, p = 0.14, Fstimulus(3, 108) = 98.94, p < 0.0001, Fgenotype/treatment(3, 36) = 0.66, p = 0.58. PPI: Finteraction(6, 74) = 0.90, p = 0.5, Fstimulus(2, 74) = 87.82, p < 0.0001, Fgenotype/treatment(3, 37) = 5.2, p = 0.0041, two-way RM ANOVA). D TORM impairments is reversed by CNO activation of BF cholinergic neurons in ChatCRECul3F/+ mice (Cul3F/+: n = 10, ChatCRECul3F/+: n = 11, Finteraction(1, 38) = 7.92, p = 0.0077; Fgenotype(1, 38) = 16.73, p = 0.0002; Fgenotype/treatment(1,38) = 8.25, p = 0.0066, two-way ANOVA, Tukey’s and Šidák’s post-hoc tests were used for multiple comparisons). In all figures, **p < 0.01; ***p < 0.001.

Back to article page