Fig. 3: Human FKBP5 mRNA expression, methylation, and hydroxymethylation analysis.

a Schematic representation of the human FKBP51 gene proximal promoter region indicating Transcription start site (TSS). The analyzed region −266 to +73 (340 bp) which includes 39 CpGs (depicted in red uppercase letters). The numbering is relative to the TSS. Underlined/italicized letters indicate the exon, and bold/black letters indicate primers used for meDIP/hmeDIP-qPCR analysis. b Relative expression levels of FKBP5 mRNA in PFC (FC = 1.61, p = 0.0009), and c hippocampus (FC = 1.36, p = 0.02). Data are expressed as fold change (FC) ± SEM (error bars) after normalization to the geometric mean of internal controls and suicide-completers compared to normal control subjects. d Pearson’s correlation analysis of FKBP5 and GR-P mRNA levels in the PFC. e and f In the PFC, 5mC, and 5hmC levels across the TSS region of FKBP5 gene analyzed using meDIP and hmeDIP and presented as percent of input. e %5mC levels (t = 3.22, p = 0.002) and f %5hmC (t = −0.25, p = 0.02). Results are expressed: %(5mC/5hmC) relative to input ± SD (error bars) measured across each region comparing suicide-completers (T-SC) to normal-control (T-NC) subjects. N = 24/24. *p < 0.05, **p < 0.01.