Fig. 5: Effects of repeated maternal separation (RMS) on regional brain volumes.

Animals underwent MRI scanning on PND 20 (RMS n = 17, control n = 12), PND 62 (RMS n = 22, control n = 20), and PND 285 (RMS n = 21, control n = 23), and data were analyzed to quantify the volumes of six regions of interest. Maternal separation resulted in increased grey matter volume in the amygdala at the timepoint in late adulthood during adult stress, but not in early adulthood or immediately following RMS.