Fig. 4: Aspirin modulates intrinsic excitability of dentate gyrus (DG) granule neurons through PPARα. | Translational Psychiatry

Fig. 4: Aspirin modulates intrinsic excitability of dentate gyrus (DG) granule neurons through PPARα.

From: Peroxisome proliferator-activated receptor-α activation facilitates contextual fear extinction and modulates intrinsic excitability of dentate gyrus neurons

Fig. 4

a Action potential (AP) trains evoked by step-current injections in DG granule neurons of WT mice treated with aCSF (black) and 100 μM aspirin at 5 min (pink) or 30 min (red). b Number of Aps in aspirin treated DG granule neurons (treatment: F (2,180) = 3.023, p = 0.0511; current: F (9,90) = 24.41, p < 0.0001; treatment × current: F (18,180) = 4.349, p < 0.0001). *p < 0.05 compared with ASP5; ##p < 0.01 and ####p < 0.0001 compared with ASP30. c Rheobase current (F (2, 18) = 7.277, p = 0.0048). d Input resistance (IR). Left, input resistance (F (2, 18) = 14.38, p = 0.0002). Right, IR vs rheobase of DG granule neurons. e Resting membrane potential. Left, resting membrane potential (F (2, 18) = 4.081, p = 0.0346). Right, RMP vs rheobase of DG granule neurons. f Representative action potential waveform during ramp depolarization in DG granule neurons of WT mice treated with aCSF (black) and 100 μM aspirin at 5 min (pink) or 30 min (red). g AP amplitude (F (2, 18) = 1.005, p = 0.3857). h AP half-width (F (2, 18) = 4.206, p = 0.0317). i Post-burst afterhyperpolarization (AHP) (F (2, 18) = 6.014, p = 0.01). n = 10 neurons from 3 mice. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with aCSF. j Action potential (AP) trains evoked by step-current injections in DG granule neurons of PPARα−/− mice treated with aCSF (red) and 100 μM aspirin at 5 min (blue) or 30 min (navy). k Number of APs (treatment: F (2, 160) = 2.060, p = 0.1308; current: F (9, 80) = 5.849, p < 0.0001; treatment × current: F (18, 160) = 0.4932, p = 0.9582). l Rheobase current. F (2, 16) = 0.04061, p = 0.9603. m Input resistance. Left, input resistance (F (2, 16) = 5.205, p = 0.0182). Right, IR vs rheobase of DG granule neurons. n Resting membrane potential. Left, resting membrane potential (F (2, 16) = 0.4154, p = 0.6670). Right, RMP vs rheobase of DG granule neurons. o Representative action potential waveform during ramp depolarization in DG granule neurons treated with aCSF (red) and 100 μM aspirin at 5 min (blue) or 30 min (navy). p AP amplitude (F (2, 16) = 2.739, p = 0.0949). q AP half-width (F (2, 16) = 2.441, p = 0.1188). r post-burst afterhyperpolarization (AHP) (F (2, 16) = 2.283, p = 0.1342). n = 9 neurons from 3 mice. *p < 0.05 compared with aCSF. Data are presented as mean ± s.e.m.

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