Fig. 3: Effect of Mbd2 deficiency on gene expression and DNA methylation.
From: Methyl-CpG binding domain 2 (Mbd2) is an epigenetic regulator of autism-risk genes and cognition
A Heatmap of all transcribed genes in Mbd2−/− and wild-type mice sorted by log2 fold-change. B Pathway analysis enrichment of downregulated genes (Top 5 pathways are presented). C Pathway analysis enrichment of upregulated genes (Top 5 pathways are presented). D A cumulative distribution of methylation levels of regulatory DNA regions bound by Mbd2 demonstrating hyper-methylation in Mbd2−/− hippocampus (K-S test). E Number of differentially methylated CpGs (with at least 10% change in methylation and p < 0.001) in Mbd2-bound annotated genes showing that more CpGs became hyper-methylated then hypo-methylated (p = 0.00339, binomial test). F Number of differentially methylated CpGs (with at least 10% change in methylation and p < 0.001) located within Mbd2-peaks (p < 1E-8, binomial test). G Pearson correlation between differential promoter DNA-methylation and gene-expression in Mbd2−/− hippocampi.
