Fig. 3: Pathological analysis of PV neurons constituting the 3q29-del brain via immunostaining.
From: Phenotypes for general behavior, activity, and body temperature in 3q29 deletion model mice
The immunohistochemistry of the brains of adult 3q29-del and WT mice. The whole brains of the mice were thinly sliced (coronal) and immunostained with anti-Parvalbumin (PV) antibodies. The fluorescence of the whole sections was observed, and the granular signals were measured in four regions with the help of Fiji (Image J2): cortex, hippocampus, thalamus, and hypothalamus. Furthermore, the number of signals per mm2 was compared between the WT (a) and 3q29-del (b) mice, and statistical analysis was performed using Student’s t-test (c). Bars = 1 mm.
