Fig. 2: Overexpressing APP promotes SARS-CoV-2 pseudovirus infection in HEK293T and SH-SY5Y cells. | Translational Psychiatry

Fig. 2: Overexpressing APP promotes SARS-CoV-2 pseudovirus infection in HEK293T and SH-SY5Y cells.

From: Amyloid precursor protein facilitates SARS-CoV-2 virus entry into cells and enhances amyloid-β-associated pathology in APP/PS1 mouse model of Alzheimer’s disease

Fig. 2

A and B APP facilitates pseudovirus (Pseudo-SARS-CoV-2 virus) infection. A HEK293T, HEK293T/hACE2, and HEK293T/hACE2 transiently transfected with constructs encoding APP (HEK293T/hACE2 transient with APP) were plated in six-well chambers at a density of 1 × 105/well for 24 h. Cells were infected with the GFP-labeled pseudovirus at 108/mL for 48 h at 37 °C, and visualized by fluorescence microscopy. Scale bar 100 μm. B HEK293T/hACE2 cells transfected with constructs encoding hAPP or HEK293T/ hAPP cells transfected with constructs encoding hACE2 were infected with the GFP-labeled pseudovirus at 108/mL for 72 h at 37 °C. Pseudovirus mRNA from cell lysis was examined by RT-qPCR. The data are shown as the means ± SEM from three independent experiments. P values were calculated using two-way ANOVA (*P < 0.05; ***P < 0.001). C SH-SY5Y cells, SH-SY5Y cells stably overexpressing hAPP and SH-SY5Y cells with APP knockdown were infected with the GFP-labeled pseudovirus at 108/mL and visualized by fluorescence microscopy at 48 h post-infection. Scale bar 100 μm. D Pseudovirus from cells lysis in C was examined by RT-qPCR. The data are shown as the means ± SEM from three independent experiments. P values were calculated using two-way ANOVA (***P < 0.001). E The APP overexpression and knockdown efficiencies in SH-SY5Y were evaluated using WB assay with anti-APP antibody (6E10). GAPDH was selected as a reference control.

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