Fig. 4: Enhanced GABAergic tone from the RMTg during conditioning to cocaine attenuates changes in GluA1ps831 but not GluA1ps845 in the PFC.
All rats were euthanized 45 min after the RePP test. Then, their PFCs were taken out and homogenized. Samples were resolved by SDS-PAGE, and WB membranes were probed with the appropriate antibodies. A The bar histograms depict the level of ERK divided by Actin, no significant difference was found (P > 0.05), as determined by a two-way ANOVA test [FOPTIC STIMULATION (1,10) = 0.003651, P > 0.05, FGROUP (1,10) = 2.211, P > 0.05, FINTERACTION (1,10) = 0.2461, P > 0.05, followed by Tukey’s multiple comparison test] (n = 3 for the saline groups, n = 4 for the cocaine groups). B The bar histograms depict the level of pERK (Thr202/Tyr204) divided by ERK protein, no significant difference was found (P > 0.05), as determined by a two-way ANOVA test [FOPTIC STIMULATION (1,10) = 0.01467, P > 0.05, FGROUP (1,10) = 4.050, P > 0.05, FINTERACTION (1,10) = 1.125, P > 0.05, followed by Tukey’s multiple comparison test]. (n = 3 for the saline groups, n = 4 for the cocaine groups). C The bar histograms depict the level of GluA1 divided by Actin, no significant difference was found (P > 0.05), as determined by a two-way ANOVA test [FOPTIC STIMULATION (1,10) = 0.04842, P > 0.05, FGROUP (1,10) = 0.7459, P > 0.05, FINTERACTION (1,10) = 0.03048, P > 0.05, followed by Tukey’s multiple comparison test]. (n = 3 for the saline groups, n = 4 for the cocaine groups). D The bar histograms depict the level of GluA1ps845 divided by GluA1 protein, rats treated with cocaine (15 mg/kg) displayed significantly higher values compared to the saline without optic stimulation and saline with optic stimulation groups (P < 0.05), no significant difference was found between the cocaine group and cocaine with optic stimulation group (P > 0.05), as determined by a two-way ANOVA test [FOPTIC STIMULATION (1,18) = 0.003881, P > 0.05, FGROUP (1,18) = 16.48, P < 0.001, FINTERACTION (1,18) = 0.0005230, P > 0.05, followed by Tukey’s multiple comparison test]. (n = 5 for the saline groups, n = 6 for the cocaine groups). E The bar histograms depict the level of GluA1ps831 divided by GluA1 protein, rats treated with cocaine (15 mg/kg) displayed significantly higher values compared to all other groups (P < 0.05 for saline- optic stimulation, P < 0.001 for saline + optic stimulation and cocaine+ optic stimulation groups), as determined by a two-way ANOVA test [FOPTIC STIMULATION (1,19) = 24.75, P < 0.0001, FGROUP (1,19) = 3.460, P > 0.05, FINTERACTION (1,19) = 10.23, P < 0.01, followed by Tukey’s multiple comparison test]. (n = 6 for the saline groups, n = 5 and 6 for the cocaine– and cocaine+ groups, respectively). (Off refers to –optic stimulation, whereas On refers to +optic stimulation).
