Table 1 Primer sequence and amplification length of destination fragment.
Gene | Number | Upstream and downstream primer sequence | Product length (bp) |
|---|---|---|---|
Panx1 | NM_001270548.1 | F: 5′-GCTGCACAAGGTAATAATGAGTCT-3′ R: 5′-AGGGCGTACACTAGGAGGTT-3′ | 333 |
P2rx7 | NM_019256.2 | F: 5′-GCTGAGAATCGGTGTGCTTTC-3′ R: 5′-CTGCAACGCCTTTGACCTTG-3′ | 316 |
Mavs | NM_001005556.1 | F: 5′-CTTTAGCAAGCAGTCCATCTCT-3′ R: 5′-TGCTTGTAGGAAGCCCGTAA-3′ | 218 |
Txnip | NM_001008767.2 | F: 5′-TAGTGATTGGCAGCAGGTCG-3′ R: 5′-CTCAGTGTAAGTGGGCGGAG-3′ | 260 |
Cathepsin B | NM_022597.2 | F: 5′-CAGGCTGGACGCAACTTCTA-3′ R: 5′-CAGGTAAGCAGGTCCTCAGC-3′ | 299 |
Nek7 | NM_006249926.3 | F: 5′-ATGGATGAACAACCACAAGGAAT-3′ R: 5′-TGGAACTCCATCCAGGAGACA-3′ | 177 |
GAPDH | NM_017008.4 | F: 5′-AGTGCCAGCCTCGTCTCATA-3′ R: 5′-GATGGTGATGGGTTTCCCGT-3′ | 248 |
