Fig. 6: Acute PLX3397 treatment promotes clearing of Aβ-loaded glutamatergic synapses in both the CA1 and CA2 hippocampal regions.
A Hippocampal areas where the amyloid staining (red) is more evident are indicated (white arrows) in slices incubated with Ab1–42. B The vGlut1 (green) and amyloid (red) double immunofluorescent staining shows no clearly detectable amyloid at the vGlut1 glutamatergic terminals of Aβ1–42 incubated hippocampal slices. C The vGlut1 (green) and amyloid (red) double immunofluorescent staining in the CA1 region of the hippocampus of the Aβ1–42 and in the Aβ1–42 + PLX groups is shown at low (upper image) and high (islet) magnifications. D The vGlut1 (green) and amyloid (red) double immunofluorescent staining in the CA2 region of the hippocampus of the Aβ1–42 and in the Aβ1–42 + PLX groups is shown at low and high (islet) magnifications. E The number of amyloid/vGlut1 colocalization spots is significantly augmented following amyloid incubation (Aβ1–42 group, **p < 0.01), as compared to both vehicle and PLX3397 alone (PLX) but normalizes to control level when amyloid is co-incubated with PLX3397 (Aβ1–42 + PLX). F The area of amyloid/vGlut1 colocalization spots follows the same trend, with a significant increase in the Aβ1–42 group, as compared to vehicle (*p < 0.05), Aβ1–42 + PLX (*p < 0.05), and PLX (*p < 0.05) groups. G The number and H the area of vGlut1-positive spots are comparable in all the experimental groups analyzed (p = 0.5834 and p = 0.3427, respectively; n=3/group). Arrowheads point to the vGlut1/amyloid colocalization puncta in both the cell body and dendritic layers of the CA1 and CA2. DAPI (blue) was used to counterstain nuclei. CA1-3 Cornu Ammonis 1-3, DG dentate gyrus. Scale bars: A 200 μm; B 100 μm; C 50 μm; islet, 10 μm; D 50 μm; islet, 30 μm. SO stratum oriens, PL stratum pyramidale, SR stratum radiatum, CA2 Cornu Ammonis 2.
