Fig. 1: Experimental timeline of long-term alcohol consumption and ECoG recordings.
a In alternating cycles, animals had access to either alcohol solutions of 5%, 10%, and 20% ethanol besides water (free access to alcohol) or to water alone (deprivation). Alcohol consumption phases were initially 8 weeks and then between 4 – 6 weeks, interspersed with deprivation periods of 2 – 3 weeks. Following the final (8th) alcohol-drinking cycle, rats were habituated to the recording set-up and underwent stereotactic surgery to implant the neuroprosthetic interface. Alcohol-detoxified animals underwent electrocorticographic (ECoG) recordings during a two-tone auditory oddball paradigm initially three days post-surgery without any further interventions. Subsequent recording sessions were performed 30 min following i.p. administration of psilocybin or LY379268 in a randomized order nine- and twelve-days post-surgery, respectively. The auditory oddball paradigm consisted of altogether 1620 tones (1344 standards, 276 deviants), delivered in 6 × 5 min blocks with varying breaks (<1 min) between blocks. b Alcohol consumption patterns throughout the experimental timeline. Data are presented as individual data points (grey) as well as daily means ± standard error of the mean of pure EtOH in g per kg over all animals (black) during the last week of a drinking phase (baselines, BL) and on the first day following periods of abstinence (i.e. Alcohol Deprivation Effect (ADE)).
