Fig. 2: The social stress induces the mutual innervation between auditory cortex (ACtx) and trunk area of somatosensory cortex (S1-Tr).
A Retro-AAVs-mCherry are microinjected in the S1-Tr cortex. B The retrogradely traced neurons in the auditory cortex (post bregma 2.30 mm, scale bar, 50 µm) in samples of control mouse (left panel) and intruder mouse (right panel). C Statistical analyses show mCherry-labeled cells per mm3 in the auditory cortices from control mice (blue symbols) and intruder mice (red symbols; ***, P < 0.001, one-way ANOVA). D Retro-AAVs-mCherry are microinjected in the auditory cortex. E The retrogradely traced neurons in the S1-Tr cortex (post bregma 1.70 mm, scale bar, 50 µm) in samples of control mouse (left panel) and intruder mouse (right panel). F Statistical analyses show mCherry-labeled cells per mm3 in the S1-Tr cortices from control mice (blue symbols) and intruder mice (red symbols; *, P < 0.05, one-way ANOVA). G An image shows the injection site of retro-AAVs-mCherry in the auditory cortex. H An immunofluorescent staining image with low power, in which the white frame shows the S1-Tr cortex. I Images with high power in the S1-Tr cortices from control mice (top panels) and intruder mice (bottom panels). The images show DAPI-labeled neurons, mCherry-labeled neurons, cFos-labeled neurons and their merges, respectively, in left-to-right panels. J mCherry-labeled neurons in the S1-Tr cortex are significantly higher in intruder mice (red symbols) than in control mice (blue symbols; *, P < 0.05, one-way ANOVA). K cFos-labeled neurons in the S1-Tr cortex in control mice (blue symbols) and intrude mice (red symbols). L The neurons labeled by both mCherry and cFos in the S1-Tr cortex are higher in intruder mice (red symbols) than control mice (blue symbols; ***, P < 0.001, one-way ANOVA). Error bars indicate SEM.
