Fig. 3: Large-scale functional gradients in mice.

a The principal (top) and secondary (bottom) functional gradients are projected onto the brain surface mesh. b The explanation ratio of connectome gradients (components) derived from the template FC. c Scatter plots of whole-brain voxel-wise gradient score for each of the two functional gradients across different groups. Each dot represents a voxel, colored according to its functional system assignment. d The whole-brain voxel-wise gradient dispersion is violin plotted for each group. Each dot represents a single voxel. Asterisks indicate the significance of paired t-tests after adjusting for multiple comparisons (*p < 0.05, **p < 0.01, ***p < 0.001). e System-level distributions of the principal gradients for the 2-week group pair (left) and 4-week group pair (right). Bubble plots on the right represent the group differences in the gradient scores for each brain system, corrected for multiple comparisons (FWE-corrected at p < 0.05). The color of the circle represents the T-value of the paired t-test (red: SSCE > CON, blue: SSCE < CON). The size of the circle indicates the significance of the corresponding paired t-test. Radar plots of the T-values are also provided. f Pairwise comparison of within-network gradient dispersion for the 2-week group pair (left) and 4-week group pair (right). g Scatter plots of voxel-wise gradient scores of the PFC for the first two functional gradients in different groups. Each dot represents a voxel, colored according to its regional assignment. h The voxel-wise gradient dispersion in the PFC is violin plotted for each group. Each dot represents a single voxel. Asterisks indicate the significance of paired t-tests after adjusting for multiple comparisons (*p < 0.05, **p < 0.01, ***p < 0.001, FWE-corrected).