Fig. 1: Distribution and myelination of noradrenergic fibers in medial forebrain bundle.

A Noradrenalin (NA) fibers – identified using Dopamine-ß-Hydraxylase (DßH) as the marker - distribution at AP −2.76 mm (stained by AF488, show in yellow) and traced yellow pixels from a representative Sprague Dawley (SD) and Flinders sensitive line (FSL) rats. B Heat map for the distribution of NA fibers in medial forebrain bundle at different AP levels from SD rats (n = 6) and FSL rats (n = 6). C The percentage of nonzero yellow pixel in 750 ×950 pixels atlas at each AP levels. D The difference of amount of NA fibers between SD and FSL rats, Student’s t-test, t = 9.067, df = 10, P < 0.0001. E Myelination of NA fibers in medial forebrain bundle in SD rats at −2.76 mm AP. F Myelination of NA fibers in medial forebrain bundle in FSL rats at −2.76 mm AP. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Error bars: SEM. Abbreviations: APM-AHA = Area preoptica medialis - Area hypothalamica anterior, ARH = Nucleus arcuatus hypothalami and eminentia mediana, BST = Nucleus interstitialis of the stria terminalis, ci = capsula interna, CM = Corpus mamillare, DMH = Nucleus dorsomedialis hypothalami, fx = fornix, GP = globus pallidum, H1, 2 = Fields of Forel, mt = Fasciculus mamillo-thalamicus, NHA = Nucleus hypothalamicus anterior, NOT = Nucleus tractus olfactorius lateralis, pc = Pedunculus cerebri, PH = Nucleus posterior hypothalami, PMV = premammillary nucleus, ventral, SNR = Substantia nigra, pars reticulata, st = stria terminalis, SUM = Area supramamillaris, SUT = Nucleus subthalamicus, to = tractus opticus, VMH = Nucleus ventromedialis hypothalami, ZI = Zona incerta.