Fig. 3: Summary measures as markers of a chronic stress state.

A Socially or isolation-reared animals underwent three baseline and one aversive sampling events of the EPM and LD tests, then summary measures of different complexities were calculated. B-E-H SiMs (scaled and inverted time and frequency-based anxiety scores) were calculated from each sampling event using the EPM (B) or LD tests (E), or their composite score (COMP) (H). C-F-I SiMs (1 test event) were calculated from the first, while SuMs were calculated from multiple averaged sampling events (1-2, 1-2-3, 1-4), using the EPM (C), LD (F), or their composites (I). D-G-J The effect sizes of isolation rearing based on averaged sampling events (SuMs) compared to the averages of effect sizes of those very same sampling events without summarization. Gray and colored bars represent the effect sizes of the comparisons from the first (B-E-H) and the second columns (C-F-I), respectively. Experiments in this figure were done using male Wistar rats. All statistical results are presented in Table 1. * significant difference after FDR correction.