Fig. 3: TSPO Expression in Brain and Activation of ACBD3/PKA-CaMK Pathway. | Translational Psychiatry

Fig. 3: TSPO Expression in Brain and Activation of ACBD3/PKA-CaMK Pathway.

From: Critical role of translocator protein (TSPO) in neuronal mitochondrial dysfunction and mental stress-exacerbated ischemic injury following stroke

Fig. 3

A Translocator protein (TSPO) was screened through the DisGeNet and MitoCarta3.0 database. B TSPO mRNA and protein expression were analyzed 24 h after CRS in the brain, and both mRNA and protein expression level of TSPO were increased upon CRS. C Green fluorescence, representing the expression of TSPO, and red fluorescence, representing the expression of NeuN (a neuronal marker) were co-localized, and the number of TSPO+NeuN+cells was increased upon CRS in the brain (p < 0.001). D SPT ratio was negative correlated with TSPO expression. E Molecular docking prediction and visualization of binding interactions between TSPO and ACBD3. F The interaction between TSPO and ACBD3 was enhanced under CRS (p < 0.001). (G) CRS increased the expression of ACBD3 (p < 0.001) PKA (p < 0.01), and CaMK (p < 0.01), and phosphorylation of VDAC was increased upon CRS by Western blot and co-immunoprecipitation (p < 0.001). H Fluorescence intensity of PKA was increased upon CRS (p < 0.001). Data are presented as mean ± SEM.

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