Fig. 3: Perampanel has different effects in stress-susceptible and Naïve mice.
A Western blotting analysis of the Gri2B in mPFC of Naïve, Sus and SP0.5 group. (Naïve: n = 6; Sus: n = 5; SP0.5: n = 5, one-way ANOVA: Naïve vs. Sus, **p = 0.0055, Naïve vs. SP0.5, p > 0.9999, Sus vs. SP0.5, **p = 0.0031; F (2, 13) = 10.84). B Basal mPFC Grin2b/GluA1 expression in naïve vs. Sus mice pre-treatment. C Schematic of perampanel injection after 1 h and behavioral testing. D Western blotting analysis of the Gria1 in mPFC of Naïve and NP0.5 group. (Naïve: n = 4; NP0.5: n = 4, unpaired t test: *p = 0.0325, t = 2.769). E Western blotting analysis of the Grin2b in mPFC of Naïve and NP0.5 group. (Naïve: n = 4; NP0.5: n = 4, unpaired t test: **p = 0.0042, t = 4.481). F Social interaction ratio in social interaction test of Naïve and NP0.5 group mice. (Naïve: n = 6; NP0.5: n = 6, welch’s t test: *p = 0.0105, t = 3.796). G Immobility time in force swimming test of Naïve and NP0.5 mice. (Naïve: n = 6; NP0.5: n = 6, unpaired t test: **p = 0.0074, t = 3.345). H Immobility time in tail suspension test of Naïve and NP0.5 mice. (Naïve: n = 6; NP0.5: n = 6, unpaired t test: **p = 0.0068, t = 3.397). I Total distances in open field test of Naïve and NP0.5 group mice. (Naïve: n = 6; NP0.5: n = 6, unpaired t test: **p = 0.0068, t = 3.397). J Schematic diagram of conditioned place preference; Preference ratio in conditioned place preference of Naïve and NP0.5 group mice. (Naïve: n = 9; NP0.5: n = 9, paired t test, p = 0.3867, t = 0.9480). K Percentage of prepulse inhibition of the auditory startle reflex across different prepulse intensities. (Naïve: n = 6; NP0.5: n = 6, unpaired t test: The auditory startle reflex across different prepulse intensities, unpaired t test: 74 dB, p = 0.8901, t = 0.1418; 78 dB, p = 0.4322, t = 0.8183; 86 dB, p = 0.4824, t = 0.7295; 82 dB, p = 0.5523, t = 0.6144; 90 dB, p = 0.9163, t = 0.1078).
