Table 1 QPCR primer sequences.
From: 4-Methoxydalbergione is a potent inhibitor of human astroglioma U87 cells in vitro and in vivo
Gene | Primer sequence | Annealing T (°C) |
|---|---|---|
GAPDH | 5′-GTCTCCTCTGACTTCAACAGCG-3′ | 59.5 |
5′-ACCACCCTGTTGCTGTAGCCAA -3′ | 59.5 | |
BCL-2 | 5′-TGTGGATGACTGAGTACCTGAAC-3′ | 57.8 |
5′-AGACAGCCAGGAGAAATCAAA-3′ | 53.7 | |
IL-6 | 5′-AGACAGCCACTCACCTCTTCAG-3′ | 59.5 |
5′-TTCTGCCAGTGCCTCTTTGCTG -3′ | 59.5 | |
ATF4 | 5′-TCAAACCTCATGGGTTCTCC-3′ | 55.4 |
5′-GTGTCATCCAACGTGGTCAG -3′ | 57.4 | |
SESN2 | 5′-AGATGGAGAGCCGCTTTGAGCT-3′ | 59.5 |
5′-CCGAGTGAAGTCCTCATATCCG -3′ | 59.5 | |
CCNB1 | 5′-TGTTGGTTTCTGCTGGGTGT-3′ | 55.4 |
5′-TGCCATGTTGATCTTCGCCT -3′ | 55.4 | |
HSPB1 | 5′-CTGACGGTCAAGACCAAGGATG-3′ | 59.5 |
5′-GTGTATTTCCGCGTGAAGCACC -3′ | 59.5 | |
IL-1A | 5′-TGTATGTGACTGCCCAAGATGAAG -3′ | 57.9 |
5′-AGAGGAGGTTGGTCTCACTACC -3′ | 59.5 | |
CCNA2 | 5′-CAACCAGAGCAGCACAAGTAGC-3′ | 59.6 |
5′-CTGTGGTGCTTTGAGGTAGGTC -3′ | 59.5 | |
CCNB2 | 5′-CAACCAGAGCAGCACAAGTAGC-3′ | 59.5 |
5′-GGAGCCAACTTTTCCATCTGTAC -3′ | 59.8 | |
CDC20 | 5′-CGGAAGACCTGCCGTTACATTC-3′ | 59.5 |
5′-CAGAGCTTGCACTCCACAGGTA-3′ | 59.5 | |
MCM7 | 5′-GCCAAGTCTCAGCTCCTGTCAT-3′ | 59.5 |
5′-CCTCTAAGGTCAGTTCTCCACTC -3′ | 59.6 | |
PLK1 | 5′-GCACAGTGTCAATGCCTCCAAG-3′ | 59.5 |
5′-GCCGTACTTGTCCGAATAGTCC -3′ | 59.5 | |
BUB1B | 5′-GTGGAAGAGACTGCACAACAGC-3′ | 59.5 |
5′-TCAGACGCTTGCTGATGGCTCT -3′ | 59.5 | |
BUB1 | 5′-GCTCTGTCAGCAGACTTCCTTC-3′ | 59.5 |
5′-CAGCAGATGTGAAGTCTCCTGG-3′ | 59.5 | |
CDK1 | 5′-GGAAACCAGGAAGCCTAGCATC-3′ | 59.5 |
5′-GGATGATTCAGTGCCATTTTGCC -3′ | 57.8 | |
MAP2K6 | 5′-GGCTACTTGGTGGACTCTGTTG -3′ | 59.5 |
5′-CATCGTGATGCCCAGACTCCAA -3′ | 59.5 | |
RRM2 | 5′-CTGGCTCAAGAAACGAGGACTG-3′ | 59.5 |
5′-CTCTCCTCCGATGGTTTGTGTAC-3′ | 59.6 | |
p53 | 5′-TGGATGGTGGTACAGTCAGAGC -3′ | 59.5 |
5′-TGGATGGTGGTACAGTCAGAGC -3′ | 59.5 | |
BAX | 5′-TCAGGATGCGTCCACCAAGAAG-3′ | 59.5 |
5′-TGTGTCCACGGCGGCAATCATC-3′ | 59.6 |
