Fig. 8: Mitochondrial superoxide leads to dephosphorylation and mitochondrial translocation of cofilin. | Acta Pharmacologica Sinica

Fig. 8: Mitochondrial superoxide leads to dephosphorylation and mitochondrial translocation of cofilin.

From: Cepharanthine sensitizes human triple negative breast cancer cells to chemotherapeutic agent epirubicin via inducing cofilin oxidation-mediated mitochondrial fission and apoptosis

Fig. 8

MDA-MB-231 cells were treated without or with combination of cepharanthine/epirubicin in the presence or absence of MitoQ. a Whole cell lysates, cytosolic or mitochondrial fractions were prepared and subjected to Western blot using antibodies against phospho-cofilin (Ser3) and cofilin. b Representative images of confocal microscopy which showed the colocalization of cofilin (green) and MitoTracker (red). Scale bars, 10 μm. c The Pearson’s correlation coefficient (R2) of cofilin and MitoTracker colocalization was from 50 cells of five independent experiments. d Mitochondrial length was measured with ImageJ software. 50 cells of 5 independent experiments. e, f apoptosis was determined by Annexin V-FITC/PI staining and flow cytometry. g Western blot was performed to determine the expression of PARP, cleaved-PARP (CF), C-caspase 3 and cytochrome c. Data represented as mean ± SD (n = 5, ***P < 0.001, Student’s two-tailed unpaired t-tests).

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