Fig. 5: TMZ promoted the nuclear translocation and binding of Akt and HSF1. | Acta Pharmacologica Sinica

Fig. 5: TMZ promoted the nuclear translocation and binding of Akt and HSF1.

From: Trimetazidine enhances myocardial angiogenesis in pressure overload-induced cardiac hypertrophy mice through directly activating Akt and promoting the binding of HSF1 to VEGF-A promoter

Fig. 5

a Western blotting analysis of the phosphorylation of Akt and HSF1 in the cytoplasm and nuclear of HUVECs exposed to PE and TMZ with or without Akt inhibitor (AZD5363), GAPDH was used as a total and cytoplasm loading control, Lamin A/C was used as a nuclear loading control. b Quantitative analysis of Western blotting images in (a). c Immunofluorescence staining with the p-Akt antibody (red) and HSF1 (green) of HUVECs. d Quantitative analysis of immunofluorescence intensity in indicated line. e, f Immunoprecipitation assays showing the interactions between p-Akt1 and HSF1 in HUVECs. Nuclear extracts and cytosol of HUVECs were obtained from indicated groups. Ctrl PBS group, PE Norepinephrine group, PT PE + TMZ group, cells treated with PE and then treated with TMZ, PTHi PE + TMZ + HSFi group, cells were treated with HSF1 inhibitor (KRIBB11), TMZ and PE, PTAi PE + TMZ + Akti group, cells were treated with Akt inhibitor (AZD5363), TMZ and PE. Data were analyzed by two-way ANOVA analysis using GraphPad Prism 8 software (*P < 0.5; **P < 0.01; ***P < 0.01 vs. indicated group).

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