Fig. 2: Ingenuity Pathway Analysis (IPA) using Affymetrix Clariom S human chips normalized microarray-based gene expression data. | Acta Pharmacologica Sinica

Fig. 2: Ingenuity Pathway Analysis (IPA) using Affymetrix Clariom S human chips normalized microarray-based gene expression data.

From: Cynaropicrin disrupts tubulin and c-Myc-related signaling and induces parthanatos-type cell death in multiple myeloma

Fig. 2

a Illustration of the top “biological functions” and “diseases”. b Canonical pathways. A cut-off threshold (–log (P value)) of 1.3 is considered as a default parameter. Only significant biological functions and diseases as well as canonical pathways with P < 0.05 are illustrated. c Prediction of a functional network by IPA of cynaropicrin-treated AMO1 cells. The red circles emphasize the downregulation of c-MYC, STAT3, and MAPKs. In addition, PI3K and TUBA appeared to be strongly involved within this pathway disturbed by cynaropicrin. Relative changes in gene expression profiles are displayed by a color-coding system: red represents upregulated genes and green depicts downregulated genes. d The expression levels of AKT, STAT3, c-MYC, and MAP2K2 upon treatment of AMO1, L363, NCI-H929, and OPM2 with the corresponding IC50 value of cynaropicrin were determined by qRT-PCR. The X-axis represents the previously mentioned genes; however, the Y-axis shows the fold change.

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