Fig. 5: Disorganization of the microtubule distribution upon treatment with cynaropicrin. | Acta Pharmacologica Sinica

Fig. 5: Disorganization of the microtubule distribution upon treatment with cynaropicrin.

From: Cynaropicrin disrupts tubulin and c-Myc-related signaling and induces parthanatos-type cell death in multiple myeloma

Fig. 5

a U2OS cells stably express GFP-α-tubulin protein. Micrographs of U2OS cells fixed with 4% paraformaldehyde were photographed 24 h post-treatment with DMSO, 1.8 µM and 3.6 µM of cynaropicrin, 1 µM vincristine, or 1 µM paclitaxel. Cells nuclei were stained with DAPI (blue). The peripheral microtubule masses are represented by the white arrows. Images were taken with an AF7000 widefield fluorescence microscope at 40 × magnification (scale bars = 10 µm). b The living AMO1 cells were stained with Tubulin Tracker™ Deep Red. Micrographs of AMO1 cells were photographed 24 h post-treatment with DMSO, 1.8 µM and 3.6 µM of cynaropicrin. Cells nuclei were stained with Hoechst 33342 Nuclear Stain (blue). Images were taken with an AF7000 widefield fluorescence microscope at 40 × magnification (scale bars = 7 µm).

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