Fig. 6: HNF1A regulates the SHH signaling pathway and ABCB1 expression. | Acta Pharmacologica Sinica

Fig. 6: HNF1A regulates the SHH signaling pathway and ABCB1 expression.

From: Targeted inhibition of the HNF1A/SHH axis by triptolide overcomes paclitaxel resistance in non-small cell lung cancer

Fig. 6

a, b ELISAs were used to detect the concentration of SHH in the supernatant of HNF1A-knockdown and -overexpression cells. c, d RT‒qPCR and Western blotting were used to detect the mRNA and protein levels of the indicated genes after HNF1A knockdown. e, f Total RNA and protein were isolated from cells transfected with HNF1A-expressing plasmid. RT‒qPCR and Western blotting were used to evaluate the expression levels of the indicated genes. g Immunofluorescence was used to assess the location and abundance of ABCB1 protein in HNF1A-knockdown A549/PR cells. h Western blot analysis of the effect of HNF1A knockdown on the Hedgehog pathway and ABCB1 protein expression in SHH-overexpressing cells. Data are presented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. TPL triptolide, PR paclitaxel resistance.

Back to article page