Fig. 3: NGR1 remodels the intestinal gene expression profile, downregulates pro-inflammatory genes, and promotes the repair of epithelial injury. | Acta Pharmacologica Sinica

Fig. 3: NGR1 remodels the intestinal gene expression profile, downregulates pro-inflammatory genes, and promotes the repair of epithelial injury.

From: Notoginsenoside R1 promotes Lgr5+ stem cell and epithelium renovation in colitis mice via activating Wnt/β-Catenin signaling

Fig. 3

a Volcano plots of RNA-sequencing of differentially expressed genes in the colon. b Venn diagram of the intersection of Control, DSS and DSS + NGR1 group. c GO analyses showing up/down-regulated gene clusters after NGR1 treatment. d Heatmaps of inflammation and proliferation-related genes (n = 5 per group). e RT-qPCR analysis of the expression of inflammatory mediators of COX-2, iNOS, and IL-6 in the colon samples of mice. Each bar represents the mean ± SD (n = 6 per group). f Representative images of scratch wound assays for NCM460 cell monolayers at 0 and 24 h after scratching. Each bar represents the mean ± SD (n = 3 per group). g Representative BrdU immunohistochemical-stained sections of colonic tissue (scale bar = 200 μm). Each bar represents the mean ± SD (n = 3 per group). h Effect of NGR1 on the viability of NCM460 cells (n = 3 per group). i The apoptosis of NCM460 cells was detected by flow cytometry with annexin V-FITC/PI double staining, and the experiment was repeated three times. Each bar represents the mean ± SD (n = 3 per group). ###P < 0.001 vs. Control group; *P < 0.05, **P < 0.01, ***P < 0.001 vs. DSS group.

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