Fig. 3: Regulation of LPA-induced IL-10 expression in human melanoma cells. | Acta Pharmacologica Sinica

Fig. 3: Regulation of LPA-induced IL-10 expression in human melanoma cells.

From: LPA suppresses HLA-DR expression in human melanoma cells: a potential immune escape mechanism involving LPAR1 and DR6-mediated release of IL-10

Fig. 3

Melanoma cells were treated with 10 µM LPA. Time-dependent induction of IL-10 expression was examined by qPCR (a, e). AM095 and PTX were used to examine the Gi-coupled LPAR1 signaling (b, f), whereas DR6 was silenced by siRNA (c, g). siNC or siDR6-transfected A375 and A2058 melanoma cells were treated with 10 µM LPA or its vehicle for 12 h in the presence or absence of AM095. The level of IL-10 was determined in the cell supernatants (d, h). Statistical analysis was performed using one-way ANOVA and Dunnett’s posthoc test; n = 4–14, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs control, #P < 0.0008 vs. LPA alone.

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