Fig. 2: Generation and characterization of human CES2 liver- or intestine-specific transgene expressing mouse models. | Acta Pharmacologica Sinica

Fig. 2: Generation and characterization of human CES2 liver- or intestine-specific transgene expressing mouse models.

From: Intestinal human carboxylesterase 2 (CES2) expression rescues drug metabolism and most metabolic syndrome phenotypes in global Ces2 cluster knockout mice

Fig. 2

a Schematic structure of ApoE promoter-HCR1-driven expression cassette containing human CES2 cDNA; (b) schematic structure of Villin promoter-driven expression cassette containing human CES2 cDNA; (c) western blot analysis of crude membrane fractions of liver, kidney, small intestine (SI) and colon from wild-type, Ces2–/–, human CES2 liver transgenic (Ces2–/–A) and human CES2 intestine transgenic (Ces2–/–V) mice; human liver and human small intestinal (SI) lysates were used as positive controls and for comparison on the Western blots; (d) immunohistochemical staining of human CES2 in liver, small intestine and kidney of wild-type, Ces2–/–A and Ces2–/–V mice.

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