Fig. 2: Hyper-neddylation is observed in mouse fibrotic kidneys subjected to unilateral ureteral ligation (UUO). | Acta Pharmacologica Sinica

Fig. 2: Hyper-neddylation is observed in mouse fibrotic kidneys subjected to unilateral ureteral ligation (UUO).

From: Neddylation of RhoA impairs its protein degradation and promotes renal interstitial fibrosis progression in diabetic nephropathy

Fig. 2

a Representative HE and Masson staining images of kidney sections. n = 3 mice/group. Scale bar = 200 μm. b Immunohistochemistry of the kidney sections from mice subjected to UUO for 7 days. n = 3 mice/group. Scale bar = 200 μm. c Representative immunofluorescence images stained with NEDD8 (red), LTL (green), and DAPI (blue) of the cortical kidney sections from mice after UUO for 7 days. n = 3 mice/group. Scale bar = 200 μm. d, e Representative immunofluorescence images of the cortical kidney sections stained with NEDD8 (green), collagen III (red) or α-SMA (red), and DAPI (blue). n = 3 mice/group. Scale bar = 200 μm. f Western blot analysis of collagen I, α-SMA, NAE1, and NEDD8-cullins levels in the kidney tissues obtained from mice after UUO for 7 days. n = 3, mean ± SD, *P < 0.05, **P < 0.01.

Back to article page