Fig. 8: NAC treatment reverses the effect of circSARS-CV2-N1368 on impairment of HCMECs. | Acta Pharmacologica Sinica

Fig. 8: NAC treatment reverses the effect of circSARS-CV2-N1368 on impairment of HCMECs.

From: CircSARS-CV2-N1368 from SARS-CoV-2 impairs endothelial cell function through the upregulation of ATF7 to activate TLR4/NF-κB/ROS signaling

Fig. 8

a Detection of ROS level in circSARS-CV2-N1368 overexpressing HCMECs by using DCFH-DA probe. b Western blotting analysis of VEGFA expression, activations of e-NOS and HH3 in NAC-treated HCMECs with overexpression of circSARS-CV2-N1368. c Proliferation activity analysis of HCMECs by EdU assay. d, e Migration activity analysis of HCMECs was evaluated by trans-well migration assay and wound healing assay, respectively. f NAC rescued matrigel tube formation of HCMECs with overexpression of circSARS-CV2-N1368. Comparisons were made with 1-way ANOVA, n = 3 per group in (a)–(f), and 2-way ANOVA, n = 3 per group in (b), The scale bar is 50 μm in (a), (c), (d), the scale bar is 100 μm in (f), the scale bar is 200 μm in (e). *P < 0.05, **P < 0.01, ***P < 0.001.

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